Review



mcherry rubicon cghl  (Addgene inc)


Bioz Verified Symbol Addgene inc is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    Addgene inc mcherry rubicon cghl
    A , B Rubicon expression is enhanced in neurons. A Representative western blot from lysates of HeLa cells or WT murine embryonic cortical neurons and probed for Rubicon. B Rubicon band intensity normalized to total protein in HeLa cells or WT neurons ( N = 3 experiments, two-tailed unpaired t test). C – E Rubicon localizes to organelles distributed throughout the soma, dendrites, and axons of primary cortical neurons. C Representative max projection of the soma of a WT cortical neuron transfected with EGFP-Rubicon. D Representative max projection of the dendrites of a WT cortical neuron transfected with EGFP-Rubicon. E Representative max projection of an axon of a WT cortical neuron transfected with EGFP-Rubicon. F , G Rubicon colocalizes with the late endosome/lysosome marker LAMP1-Halo but not with the autophagosome marker mCherry-LC3. F Single z-plane confocal images of the soma of a WT cortical neuron transfected with mCherry-LC3, LAMP1-Halo and EGFP-Rubicon. Yellow boxes indicate EGFP-Rubicon colocalizing with either LAMP1-Halo or mCherry-LC3. G Fraction of the number of EGFP-Rubicon puncta in the soma of each neuron colocalizing with LAMP1-Halo or mCherry-LC3 ( N = 3 experiments, two-tailed unpaired t test). H – K Rubicon localization to lysosomes is RAB7-dependent. H Schematics of the domain organization of Rubicon WT and Rubicon <t>CGHL</t> with the RUN, PI3K-binding domain (PIKBD) and Rubicon homology (RH) domains annotated (domains not drawn to scale). I Single z-plane confocal images of somas of neurons transfected with GFP-RAB7, LAMP1-Halo and either mCherry-Rubicon WT or mCherry-Rubicon CGHL . Yellow boxes indicate inset regions. J Fraction of total GFP-RAB7 area in each neuronal soma colocalizing with mCherry-Rubicon WT or mCherry-Rubicon CGHL . K Fraction of total Halo-LAMP1 area in each neuronal soma colocalizing with mCherry-Rubicon WT or mCherry-Rubicon CGHL ( I – K : N = 3 experiments, two-tailed unpaired t test). All panels: Error bars indicate SEM, scale bars = 5μm. Source data are provided as a Source Data file.
    Mcherry Rubicon Cghl, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mcherry+rubicon+cghl/pmc12335601-447-20-23?v=Addgene+inc
    Average 93 stars, based on 3 article reviews
    mcherry rubicon cghl - by Bioz Stars, 2026-08
    93/100 stars

    Images

    1) Product Images from "Mitochondrial damage triggers the concerted degradation of negative regulators of neuronal autophagy"

    Article Title: Mitochondrial damage triggers the concerted degradation of negative regulators of neuronal autophagy

    Journal: Nature Communications

    doi: 10.1038/s41467-025-62379-5

    A , B Rubicon expression is enhanced in neurons. A Representative western blot from lysates of HeLa cells or WT murine embryonic cortical neurons and probed for Rubicon. B Rubicon band intensity normalized to total protein in HeLa cells or WT neurons ( N = 3 experiments, two-tailed unpaired t test). C – E Rubicon localizes to organelles distributed throughout the soma, dendrites, and axons of primary cortical neurons. C Representative max projection of the soma of a WT cortical neuron transfected with EGFP-Rubicon. D Representative max projection of the dendrites of a WT cortical neuron transfected with EGFP-Rubicon. E Representative max projection of an axon of a WT cortical neuron transfected with EGFP-Rubicon. F , G Rubicon colocalizes with the late endosome/lysosome marker LAMP1-Halo but not with the autophagosome marker mCherry-LC3. F Single z-plane confocal images of the soma of a WT cortical neuron transfected with mCherry-LC3, LAMP1-Halo and EGFP-Rubicon. Yellow boxes indicate EGFP-Rubicon colocalizing with either LAMP1-Halo or mCherry-LC3. G Fraction of the number of EGFP-Rubicon puncta in the soma of each neuron colocalizing with LAMP1-Halo or mCherry-LC3 ( N = 3 experiments, two-tailed unpaired t test). H – K Rubicon localization to lysosomes is RAB7-dependent. H Schematics of the domain organization of Rubicon WT and Rubicon CGHL with the RUN, PI3K-binding domain (PIKBD) and Rubicon homology (RH) domains annotated (domains not drawn to scale). I Single z-plane confocal images of somas of neurons transfected with GFP-RAB7, LAMP1-Halo and either mCherry-Rubicon WT or mCherry-Rubicon CGHL . Yellow boxes indicate inset regions. J Fraction of total GFP-RAB7 area in each neuronal soma colocalizing with mCherry-Rubicon WT or mCherry-Rubicon CGHL . K Fraction of total Halo-LAMP1 area in each neuronal soma colocalizing with mCherry-Rubicon WT or mCherry-Rubicon CGHL ( I – K : N = 3 experiments, two-tailed unpaired t test). All panels: Error bars indicate SEM, scale bars = 5μm. Source data are provided as a Source Data file.
    Figure Legend Snippet: A , B Rubicon expression is enhanced in neurons. A Representative western blot from lysates of HeLa cells or WT murine embryonic cortical neurons and probed for Rubicon. B Rubicon band intensity normalized to total protein in HeLa cells or WT neurons ( N = 3 experiments, two-tailed unpaired t test). C – E Rubicon localizes to organelles distributed throughout the soma, dendrites, and axons of primary cortical neurons. C Representative max projection of the soma of a WT cortical neuron transfected with EGFP-Rubicon. D Representative max projection of the dendrites of a WT cortical neuron transfected with EGFP-Rubicon. E Representative max projection of an axon of a WT cortical neuron transfected with EGFP-Rubicon. F , G Rubicon colocalizes with the late endosome/lysosome marker LAMP1-Halo but not with the autophagosome marker mCherry-LC3. F Single z-plane confocal images of the soma of a WT cortical neuron transfected with mCherry-LC3, LAMP1-Halo and EGFP-Rubicon. Yellow boxes indicate EGFP-Rubicon colocalizing with either LAMP1-Halo or mCherry-LC3. G Fraction of the number of EGFP-Rubicon puncta in the soma of each neuron colocalizing with LAMP1-Halo or mCherry-LC3 ( N = 3 experiments, two-tailed unpaired t test). H – K Rubicon localization to lysosomes is RAB7-dependent. H Schematics of the domain organization of Rubicon WT and Rubicon CGHL with the RUN, PI3K-binding domain (PIKBD) and Rubicon homology (RH) domains annotated (domains not drawn to scale). I Single z-plane confocal images of somas of neurons transfected with GFP-RAB7, LAMP1-Halo and either mCherry-Rubicon WT or mCherry-Rubicon CGHL . Yellow boxes indicate inset regions. J Fraction of total GFP-RAB7 area in each neuronal soma colocalizing with mCherry-Rubicon WT or mCherry-Rubicon CGHL . K Fraction of total Halo-LAMP1 area in each neuronal soma colocalizing with mCherry-Rubicon WT or mCherry-Rubicon CGHL ( I – K : N = 3 experiments, two-tailed unpaired t test). All panels: Error bars indicate SEM, scale bars = 5μm. Source data are provided as a Source Data file.

    Techniques Used: Expressing, Western Blot, Two Tailed Test, Transfection, Marker, Binding Assay



    Similar Products

    mcherry rubicon cghl  (Addgene inc)
    93
    Addgene inc mcherry rubicon cghl
    A , B Rubicon expression is enhanced in neurons. A Representative western blot from lysates of HeLa cells or WT murine embryonic cortical neurons and probed for Rubicon. B Rubicon band intensity normalized to total protein in HeLa cells or WT neurons ( N = 3 experiments, two-tailed unpaired t test). C – E Rubicon localizes to organelles distributed throughout the soma, dendrites, and axons of primary cortical neurons. C Representative max projection of the soma of a WT cortical neuron transfected with EGFP-Rubicon. D Representative max projection of the dendrites of a WT cortical neuron transfected with EGFP-Rubicon. E Representative max projection of an axon of a WT cortical neuron transfected with EGFP-Rubicon. F , G Rubicon colocalizes with the late endosome/lysosome marker LAMP1-Halo but not with the autophagosome marker mCherry-LC3. F Single z-plane confocal images of the soma of a WT cortical neuron transfected with mCherry-LC3, LAMP1-Halo and EGFP-Rubicon. Yellow boxes indicate EGFP-Rubicon colocalizing with either LAMP1-Halo or mCherry-LC3. G Fraction of the number of EGFP-Rubicon puncta in the soma of each neuron colocalizing with LAMP1-Halo or mCherry-LC3 ( N = 3 experiments, two-tailed unpaired t test). H – K Rubicon localization to lysosomes is RAB7-dependent. H Schematics of the domain organization of Rubicon WT and Rubicon <t>CGHL</t> with the RUN, PI3K-binding domain (PIKBD) and Rubicon homology (RH) domains annotated (domains not drawn to scale). I Single z-plane confocal images of somas of neurons transfected with GFP-RAB7, LAMP1-Halo and either mCherry-Rubicon WT or mCherry-Rubicon CGHL . Yellow boxes indicate inset regions. J Fraction of total GFP-RAB7 area in each neuronal soma colocalizing with mCherry-Rubicon WT or mCherry-Rubicon CGHL . K Fraction of total Halo-LAMP1 area in each neuronal soma colocalizing with mCherry-Rubicon WT or mCherry-Rubicon CGHL ( I – K : N = 3 experiments, two-tailed unpaired t test). All panels: Error bars indicate SEM, scale bars = 5μm. Source data are provided as a Source Data file.
    Mcherry Rubicon Cghl, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mcherry+rubicon+cghl/pmc12335601-447-20-23?v=Addgene+inc
    Average 93 stars, based on 1 article reviews
    mcherry rubicon cghl - by Bioz Stars, 2026-08
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    A , B Rubicon expression is enhanced in neurons. A Representative western blot from lysates of HeLa cells or WT murine embryonic cortical neurons and probed for Rubicon. B Rubicon band intensity normalized to total protein in HeLa cells or WT neurons ( N = 3 experiments, two-tailed unpaired t test). C – E Rubicon localizes to organelles distributed throughout the soma, dendrites, and axons of primary cortical neurons. C Representative max projection of the soma of a WT cortical neuron transfected with EGFP-Rubicon. D Representative max projection of the dendrites of a WT cortical neuron transfected with EGFP-Rubicon. E Representative max projection of an axon of a WT cortical neuron transfected with EGFP-Rubicon. F , G Rubicon colocalizes with the late endosome/lysosome marker LAMP1-Halo but not with the autophagosome marker mCherry-LC3. F Single z-plane confocal images of the soma of a WT cortical neuron transfected with mCherry-LC3, LAMP1-Halo and EGFP-Rubicon. Yellow boxes indicate EGFP-Rubicon colocalizing with either LAMP1-Halo or mCherry-LC3. G Fraction of the number of EGFP-Rubicon puncta in the soma of each neuron colocalizing with LAMP1-Halo or mCherry-LC3 ( N = 3 experiments, two-tailed unpaired t test). H – K Rubicon localization to lysosomes is RAB7-dependent. H Schematics of the domain organization of Rubicon WT and Rubicon CGHL with the RUN, PI3K-binding domain (PIKBD) and Rubicon homology (RH) domains annotated (domains not drawn to scale). I Single z-plane confocal images of somas of neurons transfected with GFP-RAB7, LAMP1-Halo and either mCherry-Rubicon WT or mCherry-Rubicon CGHL . Yellow boxes indicate inset regions. J Fraction of total GFP-RAB7 area in each neuronal soma colocalizing with mCherry-Rubicon WT or mCherry-Rubicon CGHL . K Fraction of total Halo-LAMP1 area in each neuronal soma colocalizing with mCherry-Rubicon WT or mCherry-Rubicon CGHL ( I – K : N = 3 experiments, two-tailed unpaired t test). All panels: Error bars indicate SEM, scale bars = 5μm. Source data are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: Mitochondrial damage triggers the concerted degradation of negative regulators of neuronal autophagy

    doi: 10.1038/s41467-025-62379-5

    Figure Lengend Snippet: A , B Rubicon expression is enhanced in neurons. A Representative western blot from lysates of HeLa cells or WT murine embryonic cortical neurons and probed for Rubicon. B Rubicon band intensity normalized to total protein in HeLa cells or WT neurons ( N = 3 experiments, two-tailed unpaired t test). C – E Rubicon localizes to organelles distributed throughout the soma, dendrites, and axons of primary cortical neurons. C Representative max projection of the soma of a WT cortical neuron transfected with EGFP-Rubicon. D Representative max projection of the dendrites of a WT cortical neuron transfected with EGFP-Rubicon. E Representative max projection of an axon of a WT cortical neuron transfected with EGFP-Rubicon. F , G Rubicon colocalizes with the late endosome/lysosome marker LAMP1-Halo but not with the autophagosome marker mCherry-LC3. F Single z-plane confocal images of the soma of a WT cortical neuron transfected with mCherry-LC3, LAMP1-Halo and EGFP-Rubicon. Yellow boxes indicate EGFP-Rubicon colocalizing with either LAMP1-Halo or mCherry-LC3. G Fraction of the number of EGFP-Rubicon puncta in the soma of each neuron colocalizing with LAMP1-Halo or mCherry-LC3 ( N = 3 experiments, two-tailed unpaired t test). H – K Rubicon localization to lysosomes is RAB7-dependent. H Schematics of the domain organization of Rubicon WT and Rubicon CGHL with the RUN, PI3K-binding domain (PIKBD) and Rubicon homology (RH) domains annotated (domains not drawn to scale). I Single z-plane confocal images of somas of neurons transfected with GFP-RAB7, LAMP1-Halo and either mCherry-Rubicon WT or mCherry-Rubicon CGHL . Yellow boxes indicate inset regions. J Fraction of total GFP-RAB7 area in each neuronal soma colocalizing with mCherry-Rubicon WT or mCherry-Rubicon CGHL . K Fraction of total Halo-LAMP1 area in each neuronal soma colocalizing with mCherry-Rubicon WT or mCherry-Rubicon CGHL ( I – K : N = 3 experiments, two-tailed unpaired t test). All panels: Error bars indicate SEM, scale bars = 5μm. Source data are provided as a Source Data file.

    Article Snippet: The following plasmids were used for transfections: EGFP-Rubicon (RRID: Addgene_221659), LAMP1-Halo (RRID: Addgene_221655), mCherry-LC3 (RRID: Addgene_221656), mCherry-Rubicon WT (RRID: Addgene_221657), mCherry-Rubicon CGHL (RRID: Addgene_221658) and EGFP-Rab7A (RRID: Addgene_28047), EGFP-N1 (RRID: Addgene_6085-1).

    Techniques: Expressing, Western Blot, Two Tailed Test, Transfection, Marker, Binding Assay